Lnjnbio Magnetic Beads for Polysaccharides and Polyphenols Plant Total RNA Extraction Kit231001

(Magnetic Bead Method)

【Product Introduction】

This kit uses magnetic beads and buffer system with unique separation effect to extract total RNA from roots, stems, leaves and seeds of polysaccharide and polyphenol plants, and the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The total RNA extracted is of high yield, high purity, stable and reliable quality, and can be applied to various downstream molecular biology experiments.

【Product advantages】

1, Magnetic bead extraction, without repeated centrifugation, to ensure the integrity of the extracted total RNA.

2, The extracted product has high purity, high yield and complete electrophoretic bands.

3, the use of process safety, without liquid nitrogen grinding, the product does not contain phenol, chloroform and other toxic reagents.

4, strong versatility, suitable for extracting total RNA from different kinds of polysaccharide and polyphenol plant tissue samples.

【Product image】

【Experimental case】

Case 1:Leaf samples of different polysaccharide polyphenol plants were extracted for total RNA with the following results:

No.	Sample	A260/A280	A260/A230	Conc.(ng/µL)	Yield（µg）
1	10mg Perilla leaves	1.964	1.641	71.92	7.192
2		1.965	1.653	71.92	7.192
Avg		1.9645	1.647	71.92	7.192
3	10mg Mint leaves	1.967	2.124	149.72	14.972
4		1.961	2.158	149.76	14.976
Avg		1.964	2.141	149.74	14.974
5	10mg Dandelion leaves	1.951	2.232	219.16	21.916
6		1.959	2.228	220.4	22.04
Avg		1.955	2.23	219.78	21.978

Peak pattern diagram:

Perilla leaves:

Mint leaves:

Dandelion leaves:

Total peak diagram:

The electropherogram is shown below:

 

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

The results of the ultra-micro nucleic acid assay showed that:

① The values of A260/A280 were in the range of 1.9~2.0, and the values of A260/A230 were above 1.6, and the peak shapes were complete and basically overlapped, which proved that the extracted products were of high purity.

②The yields of nucleic acids extracted from the leaves of different plants were above 7 µg.

The results of agarose gel electrophoresis showed that:

① All the samples of different polysaccharide polyphenol plant leaves have bright bands and good integrity.

In conclusion, the extracted products of different polysaccharide polyphenol plant leaf samples were of high purity, high yield and complete electrophoretic bands.

【Application area】

The extracted and purified nucleic acids from this kit can be used in a variety of downstream experiments such as RT-PCR, Real-Time PCR, microarray analysis, polyA screening, in vitro translation and molecular cloning.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.