Lnjnbio Magnetic Beads for Plant Total RNA Extraction Kit 160933

(Magnetic Bead Method) 

【Product Introduction】

Plant Total RNA Extraction Reagent is suitable for fast and efficient extraction of total RNA from plant tissue samples such as fresh plant leaves, fruits and seeds, etc. The whole process is easy to operate, especially suitable for automated nucleic acid extraction platform. During the extraction process, the sample releases nucleic acid under the action of lysis solution, and the nano-magnetic beads selectively adsorb the nucleic acid under the action of binding solution, separating the nucleic acid from other impurities in the sample; and then rinsing with washing solution to remove proteins, polysaccharides, inorganic salt ions, etc. Finally, high quality RNA can be obtained by elution, which is no need for toxic reagents such as phenol, chloroform, etc., and can be applied to various kinds of downstream molecules and can be applied to various kinds of molecules. It can be applied to various downstream molecular biology experiments.

【Product advantages】

1, Easy to operate, magnetic bead method can be used with automatic nucleic acid extractor to realize automated extraction, lysis combined with one step, efficient extraction.

2, Without repeated centrifugation, to ensure the integrity of the extracted RNA.

3, Extracted products have good purity, high yield and complete electrophoretic bands.

4, The use of process safety, without liquid nitrogen grinding, the product does not contain phenol, chloroform and other harmful reagents.

5, Strong versatility, suitable for extracting RNA from leaves, fruits, seeds and other samples of different kinds of plants.
【Product image】

 

【Experimental case】

Case 1:Fresh tender E.aureum leaves were taken for total RNA extraction experiments, and the results were as follows:

No. number	Sample intake（mg）	A260/A280	A260/A230	Conc.(ng/µL)
1	50 mg	2.087	1.579	93.85
2		2.056	1.582	103.45
3		2.099	1.477	83.6
4		2.078	1.511	85.2

The electropherogram is shown below:

 

Result analysis:

The RNA  product of E.aureum extracted with this product has high yield, complete bands and stable quality.

Case 2:Leaf samples of different plants were taken and total RNA was extracted with the following results:

No.	Sample	A260/A280	A260/A230	Conc.(ng/µL)	Yield（µg）
1	20mg Sweet potato leaves	1.972	2.039	193.76	19.376
2		1.976	2.038	194.44	19.444
Avg		1.974	2.0385	194.1	19.41
3	20mg Peach leaves	1.904	1.477	159.16	15.916
4		1.901	1.472	159.56	15.956
Avg		1.9025	1.4745	159.36	15.936
5	20mg Seasonal leaves	1.855	1.698	99.2	9.92
6		1.858	1.702	98.92	9.892
Avg		1.8565	1.7	99.06	9.906
7	20mg Eggplant leaves	1.988	1.752	98.08	9.808
8		1.987	1.755	98.6	9.86
Avg		1.9875	1.7535	98.34	9.834

Peak pattern diagram:

Sweet potato leaves:

Peach leaves:

Seasonal leaves:

Eggplant leaves:

Total peak diagram：

The electropherogram is shown below:

 

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

①The values of A260/A280 were in the range of 1.8~2.0, and the values of A260/A230 were above 1.4, and the peak shapes were complete and basically overlapped, which proved that the extracted products were of high purity.

②The yields of nucleic acids extracted from the leaves of different plants were above 9 µg.

The results of agarose gel electrophoresis showed that:

① The samples of different plant leaves have bright bands and good integrity.

In conclusion, the extracted products of different plant leaves samples were of high purity, high yield and complete electrophoretic bands.

Case 3:Leaf samples of different polysaccharide polyphenol plants were extracted for total RNA with the following results:

No.	Sample	A260/A280	A260/A230	Conc.(ng/µL)	Yield（µg）
1	10mg Perilla leaves	1.964	1.641	71.92	7.192
2		1.965	1.653	71.92	7.192
Avg		1.9645	1.647	71.92	7.192
3	10mg Mint leaves	1.967	2.124	149.72	14.972
4		1.961	2.158	149.76	14.976
Avg		1.964	2.141	149.74	14.974
5	10mg Dandelion leaves	1.951	2.232	219.16	21.916
6		1.959	2.228	220.4	22.04
Avg		1.955	2.23	219.78	21.978

Peak pattern diagram:

Perilla leaves:

Mint leaves:

Dandelion leaves:

Total peak diagram:

 

The electropherogram is shown below:

 

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

The results of the ultra-micro nucleic acid assay showed that:

① The values of A260/A280 were in the range of 1.9~2.0, and the values of A260/A230 were above 1.6, and the peak shapes were complete and basically overlapped, which proved that the extracted products were of high purity.

②The yields of nucleic acids extracted from the leaves of different plants were above 7 µg.

The results of agarose gel electrophoresis showed that:

① All the samples of different polysaccharide polyphenol plant leaves have bright bands and good integrity.

In conclusion, the extracted products of different polysaccharide polyphenol plant leaf samples were of high purity, high yield and complete electrophoretic bands.

【Application area】

The extracted and purified nucleic acids from this kit can be used in a variety of downstream experiments such as RT-PCR, Real-Time PCR, microarray analysis, polyA screening, in vitro translation and molecular cloning.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.