Lnjnbio Magnetic Beads for Plant DNA/RNA Extraction Kit 231016

(Magnetic Bead Method, No Centrifugation)

【Product Introduction】

Plant DNA/RNA extraction reagent is suitable for quickly and efficiently extracting DNA/RNA from plant tissue samples such as leaves, fruits, seeds, etc., and the whole process is convenient to operate, especially suitable for automated nucleic acid extraction platform. The extraction process releases nucleic acids under the action of the lysis buffer; Micron magnetic beads selectively adsorb nucleic acids under the action of the binding buffer, separating the nucleic acids from other impurities in the sample; Then rinse with washing buffer to remove proteins, polysaccharides, inorganic salt ions, etc.; Finally, high-quality DNA/RNA is obtained after elution. This product does not require toxic reagents such as phenol and chloroform, and the extracted DNA/RNA has high yield, high purity, stable and reliable quality, and can be applied to a variety of downstream molecular biology experiments.

【Product advantages】

1. Easy to operate, magnetic bead method can be combined with automatic nucleic acid extractor to achieve automatic extraction, one-step lysis and extraction efficiently;

2. There is no need for repeated centrifugation to ensure the integrity of the extracted DNA/RNA;

3. The extracted product has good purity, high yield and complete electrophoresis band;

4. The use process is safe, no liquid nitrogen grinding is required, and the product does not contain harmful reagents such as phenol and chloroform;

5. It is highly versatile, suitable for extracting DNA/RNA from leaves, fruits, seeds and other samples of different types of plants.

【Product image】

 

【Experimental case】

Case 1:

Table 1、DNA/RNA extraction results from fresh Brassica rapa leaves

No.	Sample	A260/A280	A260/A230	Conc.(ng/ul)
1	5mg Brassica rapa leaves A	1.973	1.971	775
2		1.944	1.925	809.6
Avg		1.9585	1.948	792.3
3	5mg Brassica rapa leaves B	1.972	2.017	743.15
4		1.922	1.816	754.1
Avg		1.947	1.9165	748.625
5	5mg Brassica rapa leaves C	2.007	2.221	711.05
6		1.942	2.087	331.25
Avg		1.974	2.154	521.15

The electropherogram is shown below:

M:Marker（2K，1K，750bp，500bp，250bp，100bp）

1-2：5mg Brassica rapa leaves A   3-4：5mg Brassica rapa leaves B   5-6：5mg Brassica rapa leaves C  

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

①The values of A260/A280 and A260/A230 are above 1.9, which proves that the extracted products are of high purity.

②Highly yields of nucleic acids extracted from Brassica rapa leaves.

The results of agarose gel electrophoresis showed that:

①All the samples of different plant leaves have bright bands and good integrity.

In conclusion, the extracted products of different plant leaves samples were of high purity, high yield and complete electrophoretic bands.

Case 2:

Table 2、DNA/RNA extraction results from fresh tobacoo leaves

Sample	No.	A260/A280	A260/A230	Conc.(ng/μL)
Fresh tobacoo leaves 10 mg	1	2.08	1.527	90.55
	2	2.075	1.52	89.85
	3	2.045	1.397	98.05
	4	2.029	1.475	102.95

The electropherogram is shown below:

Result analysis: The DNA/RNA extracted from fresh tobacco leaves with this product had good purity, high yield and intact bands.

Case 3:

Table 3、DNA/RNA extraction results from fresh pothos leaves

样本	No.	A260/A280	A260/A230	Conc.(ng/μL)
fresh pothos leaves 50mg	1	2.078	1.511	85.2
	2	2.1	1.426	99.5
	3	2.094	1.104	92.3
	4	2.1	1.499	78.5
	5	2.087	1.579	93.85

The electropherogram is shown below:

Result analysis: The DNA/RNA extracted from fresh tobacco leaves with this product had good purity, high yield and intact bands.

【Application area】

Nucleic acids purified by this kit can be used for PCR, qPCR, Enzyme digestion, library construction and Southern-Blot.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.