Lnjnbio Magnetic Beads for Plant DNA/RNA Extraction Kit 231011

(Magnetic Bead Method)

【Product Introduction】

The Plant DNA/RNA Extraction Reagent is suitable for the fast and efficient extraction of DNA/RNA from plant tissue samples such as plant leaves, fruits, seeds, etc. The whole process is easy to operate and is especially suitable for automated nucleic acid extraction platforms. The extraction process releases nucleic acid under the action of lysis solution; magnetic beads selectively adsorb nucleic acid under the action of binding solution, separating nucleic acid from other impurities in the sample; then rinsing with washing solution to remove proteins, polysaccharides, inorganic salt ions, etc.; and finally, high-quality DNA/RNA is obtained by elution.The product does not require toxic reagents such as phenol, chloroform, etc., and can be applied to many types of plant tissues. It can be applied to many kinds of downstream molecular biology experiments.

【Product advantages】

1, Easy to operate, magnetic bead method can be used with automatic nucleic acid extractor to realize automated extraction, lysis combined with one step, efficient extraction;

2, No need for repeated centrifugation to ensure the integrity of the extracted DNA/RNA;

3, Extracted products have good purity, high yield and complete electrophoretic bands;

4, The use of process safety, without liquid nitrogen grinding, the product does not contain phenol, chloroform and other harmful reagents;

5, Strong versatility, suitable for extracting DNA/RNA from leaves, fruits, seeds and other samples of different kinds of plants.

【Product image】

 【Experimental case】

Case 1:

 Concentration and purity values of Q5000 assay for fresh plant leaves

No.	Sample	Sample intake(mg)	A260/A280	A260/A230	Conc.(ng/µL)
1	Baby cabbage fresh leaves	20	2.18	2.155	226.15
2		20	2.195	2.136	232.1
3		20	2.206	2.138	227
4	Hangzhou cabbage fresh leaves	20	2.184	2.137	218.65
5		20	2.191	2.108	227.3
6		20	2.239	1.936	161.55
7	Amaranth fresh leaves	20	2.16	2.05	332.85
8		20	2.169	2.072	335.35
9		20	2.165	1.963	246.2
10	Oily lettuce fresh leaves	20	2.166	1.99	222.7
11		20	2.165	1.984	230.05
12		20	2.165	1.98	237.2
13	CorianderFresh leaves	20	2.143	1.937	137.65
14		20	2.134	1.943	139.5
15		20	2.144	1.933	141.05

The electropherogram is shown below:

Result analysis: Fresh plant leaf sample DNA/RNA extracted with this product has good purity, high yield and complete bands.

Case 2:Leaf samples of different plants were taken and DNA/RNA was extracted with the following results:

No.	Sample	A260/A280	A260/A230	Conc.(ng/µL)	Yield(µg)
1	20mg Sweet potato leaves	1.935	2.2	772.75	77.275
2		1.934	2.206	777.65	77.765
Avg		1.9345	2.203	775.2	77.52
3	20mg Castor leaves	1.928	2.148	873.45	87.345
4		1.928	2.141	877.2	87.72
Avg		1.928	2.1445	875.325	87.5325
5	20mg Lettuce leaves	1.91	2.102	209.4	20.94
6		1.91	2.105	213.7	21.37
Avg		1.91	2.1035	211.55	21.155
7	20mg Carob tree leaves	1.899	1.846	250.4	25.04
8		1.898	1.849	245	24.5
Avg		1.8985	1.8475	247.7	24.77

Peak pattern diagram：

Sweet potato leaves：

Castor leaves：

 Lettuce leaves：

Carob tree leaves：

Total peak diagram:

The electropherogram is shown below:

M:Marker（2K，1K，750bp，500bp，250bp，100bp）;

1-2： 20mg Sweet potato leaves（extracted product diluted 4 times）      

3-4： 20mg Castor leaves（extracted product diluted 5 times）

5-6： 20mg Lettuce leaves                          

7-8： 20mg Carob tree leaves

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

①The values of A260/A280 and A260/A230 are above 1.8, and the peak shapes are complete and basically coincide, which proves that the extracted products are of high purity.

②The yields of nucleic acids extracted from different plant leaf samples were above 20 µg.

The results of agarose gel electrophoresis showed that:

①All the samples of different plant leaves have bright bands and good integrity.

In conclusion, the extracted products of different plant leaves samples were of high purity, high yield and complete electrophoretic bands.

【Application area】

Nucleic acids purified by this kit can be used for PCR, qPCR, Enzyme digestion, library construction and Southern-Blot.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.