Lnjnbio Magnetic Beads for Bacteria DNA/RNA Extraction Kit 231017

(Magnetic Bead Method)

【Product Introduction】

This product uses a lysis buffer to directly lyse Gram-negative samples or lyse Gram-positive samples with lysozyme to release nucleic acids, and the magnetic beads selectively adsorb nucleic acids in the lysis buffer under the action of the binding buffer. A small amount of impurities adsorbed by the magnetic beads are removed by cleaning the washing buffer. Under the action of the elution buffer, the magnetic beads release the adsorbed nucleic acid, namely to obtain high-quality DNA / RNA. The DNA / RNA extracted from this product requires no toxic reagents such as phenol and chloroform, and the obtained DNA / RNA can be directly used for downstream experiments.

【Product advantages】

1. Before the magnetic bead method to extraction, the bacterial DNA / RNA with high purity and better integrity can be obtained without centrifugation.
2. Simple and fast, 10-30 µg of nucleic acid can be obtained from 1 mL of bacterial buffer for about 30 min.

3. Magnetic bead method extraction can process multiple samples at the same time, achieving high throughput and automation extraction.

4.The use process is safe, without liquid nitrogen grinding, the product does not contain phenol, chloroform, and other harmful reagents.

5. Proteinase K does not need to be added in the extraction proces. The kit can be transported and stored at room temperature.

6. High sensitivity, the kit can be suitable for trace sample extraction.

【Product image】

【Experimental cases】

Case 1: E. coli was grown overnight, the bacteria were collected by centrifugation, and DNA / RNA was extracted. The results were as follows:

Number	Sample	A260/A280	A260/A230	Conc.(ng/μL)	Yield(μg)
1	500 μL	1.952	2.084	57.45	5.745
2		1.956	2.052	57.35	5.735
Avg		1.954	2.068	57.4	5.74
3	600 μL	1.939	2.017	79.45	7.945
4		1.934	2.028	79.45	7.675
Avg		1.937	2.023	78.1	7.81
5	800 μL	1.953	2.110	98.6	9.86
6		1.956	2.111	96.75	9.675
Avg		1.955	2.111	97.675	9.7675

Peak pattern diagram:

Bacterial solution of 500 μL:

Bacterial solution of 600 μL:

Bacterial solution of 800 μL:

Total peak diagram:

The electropherogram is shown below:

M:Marker（2,000 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, 100 bp）;

1-2:Bacterial solution of 500 μL  

3-4:Bacterial solution of 600 μL  

5-6:Bacterial solution of 800 μL 

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay test showed that:

① The value of  A₂₆₀ / A₂₈₀ is above 1.9, and the value of A₂₆₀ / A₂₃₀ is above 2.0. The complete and basic coincidence of peak shape prove the high purity of the extraction product.

② The nucleic acid extraction yield of different E.coli samples was above 5 µg.

The results of the agarose gel electrophoresis showed that:

① Different E.coli samples have bright and good integrity bands.

In conclusion, the nucleic acid extraction yield of E.coli has high purity, high yield, complete electrophoretic band. 

【Application area】

The purified nucleic acids extracted from this kit can be used for PCR, qPCR, enzyme digestion, library construction, and Southern-Blot.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.