Animal Tissue DNA/RNA Extraction Kit 231012

This kit uses magnetic beads and buffer system with unique separation effect to extract DNA/RNA from animal tissues, the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The extracted nucleic acids are of high yield, high purity, stable and reliable quality, and can be applied to various downstream molecular biology experiments.

Product Advantages

1. Simple and fast: direct lysis, no need to incubate for long time, high quality DNA/RNA can be obtained within 30 min

2. Safe process: no liquid nitrogen grinding of samples

3. Safe and non-toxic: no organic reagents such as phenol/chloroform required

4. Wide range of applications: applicable to a wide range of animal tissues, successfully extracted from pork, beef, fish, shrimp, porcine and other samples to obtain DNA / RNA

5. High-throughput: can be used with automated nucleic acid extraction platforms to realize high-throughput extraction

Experimental Case

Case: Different tissue samples from different animals were extracted for DNA/RNA and the results were as follows:

Number	Sample	A260/A280	A260/A230	Conc.(ng/μL)	Yield(μg)	CV
1	20mgFish	1.856	1.884	109.25	10.925
2		1.866	1.956	117.4	11.74
Average		1.861	1.92	113.325	11.3325	0.28%
3	20mg Chicken meat	1.886	2.046	284.55	28.455
4		1.889	2.041	249.4	24.94
Average		1.8875	2.0435	266.975	26.6975	6.58%
5	5mg Fish liver	1.806	1.587	256.45	25.645
6		1.806	1.572	253.4	25.34
Average		1.806	1.5795	254.925	25.4925	0.60%
7	5mg Chicken heart	1.856	1.966	101.95	10.195
8		1.85	1.999	107.2	10.72
Average		1.853	1.9825	104.575	10.4575	2.51%

Peak pattern diagram:

Fish：

Chicken meat:

Fish liver：

Chicken heart：

Total peak shape：

The electropherogram is shown below:

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

①A260/A280, A260/A230, the coefficient of variation was below 6.58%, which proved the high stability of reagent extraction.

②The values of A260/A280 were above 1.8, and the values of A260/A230 were in the range of 1.5-2.0, and the peak shapes were complete and basically overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields ranged from 10-28 µg or more for different animal tissue samples.

The results of agarose gel electrophoresis showed that:

①Different tissue samples from different animals have bright bands with good integrity.

In conclusion: the nucleic acids of different animal samples were extracted by the fully automatic nucleic acid extraction and purification instrument, and the extracted products were of high purity, high yield, complete electrophoretic bands, and the reagents were uniform and stable in cooperation with the instrument.

Application Area

Nucleic acids purified by this kit can be used for PCR, qPCR, digestion, library construction and Southern blot.