Professional Manufacturer of Biomagnetic Beads
PRODUCT PARAMETERS
- Product No.: 231020M
- Extraction Methods: magnetic bead method
- Sepc: Bottled 50T/box, 100T/box
Description
(Magnetic Bead Method, Sample Volume 200-500 μL)
【Product Introduction】
This product is used for the extraction, enrichment and purification steps of genomic nucleic acids in 300-500µL fresh, frozen or anticoagulant-containing whole blood samples. The sample is lysed by guanidinium salt and nucleic acid is released. The nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed by Washing Buffer to remove protein, residual guanidinium salt, etc. Finally, the purified nucleic acid is obtained after elution. The whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The extracted genomic DNA has high yield, high purity, stable and reliable quality, and can be used in downstream molecular biology experiments such as PCR, nucleic acid detection and analysis, gene mutation detection and sequencing.
【Product advantages】
1、For different sample volume ( 300-500µL ) , can achieve good extraction results
2、High purity, high yield and complete electrophoretic bands
3、Strong versatility, suitable for extracting genomic DNA from different types of animal blood samples
4、Safe and convenient to use, no need for centrifugation, the product does not contain phenol, chloroform and other harmful reagents
5、High extraction efficiency, the whole extraction process only takes about 20 min
6、Using the magnetic bead extraction principle, it can be used with 24-channel automated nucleic acid extractor to realize the extraction of 500 µL-2 mL large-volume samples.throughput operation
【Product image】
【Experimental cases】
Case:
Whole blood genomic DNA extraction experiments were performed with the following results:
Sample number | Sample | Sample volume(µL) | A260/A280 | A260/A230 | Conc.(ng/µL) |
1 | Pig’s blood | 200 | 1.838 | 1.753 | 95.25 |
2 | 1.84 | 1.851 | 92.3 | ||
3 | 300 | 1.85 | 1.635 | 141.35 | |
4 | 1.831 | 1.846 | 135.45 | ||
5 | 500 | 1.806 | 1.731 | 239.95 | |
6 | 1.804 | 1.717 | 243.3 |
The electropherogram is as follows (gel concentration 1%, sample volume 6µL):
Result analysis: Porcine blood genomic DNA extracted with this product for various sample sizes showed good purity, high yield, complete bands, and small differences between replicates.
【Application area】
The extracted and purified nucleic acids in this kit can be used in a variety of routine experiments, such as enzyme digestion, PCR, library construction, Southern hybridization, and microarray hybridization, high-throughput sequencing.
Supplier Introduction
Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.
We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.
Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.
FAQs
We have magnetic beads in silica, magnetic beads in carboxylic acid, and various kits for nucleic acid extraction by magnetic bead method (samples can be extracted from whole blood, serum plasma free, plasmid, etc.), and the packing specifications of magnetic beads are 2ml, 10ml, 50ml, 500ml, 1000ml, etc. The packing specifications of the kits are: bottles are 50T/box, 100T/box, 200T/box, and the pre-filled kits are 32T/box, 48T/box, 64T/box, 96T/box, and 48T/box. We have 32T/box, 48T/box, 64T/box, 96T/box, and so on.
Land transportation, sea transportation, air transportation, you can choose according to your requirements.
Of course, we provide customized service and also support OEM\ODM.
First, confirm the demand, then sign the contract; after receiving the payment, we will ship the goods within the stipulated or mutually agreed delivery period.
Usually, we will send out the goods within 15 working days after receiving the payment; customized products and large quantities of orders (magnetic beads ≥ 10L, nucleic acid extraction reagents ≥ 300000T) need to be determined by both sides to communicate with the delivery period.