Lnjnbio Magnetic Beads for Stool Total DNA Extraction Kit 221005

(Magnetic Bead Method)

【Product Introduction】

This product is suitable for the rapid and efficient extraction of total DNA from stool samples. The extraction process uses Digestive Buffer and Proteinase K to lyses the intestinal flora cells in the stool, releasing the total DNA. The magnetic beads selectively adsorb the total DNA in the lysate under the action of the binding buffer. The small amount of impurities adsorbed by the magnetic beads is removed by the Washing Buffer. The magnetic beads release the adsorbed total DNA under the action of the Elution Buffer, and the high-quality total DNA of intestinal flora can be obtained. The total DNA of intestinal flora extracted by this product does not need toxic reagents such as phenol, chloroform, etc., and the total DNA obtained can be directly used in downstream experiments.

【Product advantages】

1. High extraction yield: 10-60 μg of total DNA can be extracted from 200 mg of sample.

2. Wide applicability: suitable for solid or liquid stool samples from different sources.

3. Easy to operate: the extraction process can be completed within 30 min, also suitable for high-throughput operation through the automated workstation.

4. High safety: free of toxic reagents such as phenol and chloroform.

【Product image】

【Experimental cases】

Case 1:

Total DNA was extracted from six stool samples of different sources (sampling volume of 200 mg) using theStool Total DNA Extraction Kit from Shanghai Lingjun Biotechnology Co. Ltd. on an automated nucleic acid extraction and purification instrument, LJbio32H, and about 10 μg of total DNA could be captured with high stability.

Case 2：

The same stool sample was extracted multiple times (10 repetitions) using the Stool Total DNA Extraction Kit from Shanghai Lingjun Biotechnology Co., Ltd. and quantitatively amplified by qPCR to detect 16S rRNA targets.

Result analysis：

The results showed that the Ct values for multiple extracted samples were essentially the same, thus indicating good reproducibility of the extraction kit.

【Application area】

1. The extracted and purified nucleic acids of this kit are suitable for various routine experiments such as water quality and safety testing, pathogen detection, PCR, and disease marker discovery and high-throughput sequencing.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.