Serum/Plasma Free DNA Extraction Kit(Magnetic Bead Method) 231003

【Product Introduction】

The lysis solution in this product not only plays a role in lysing samples such as serum/plasma, but also provides an environment for the magnetic beads to selectively adsorb nucleic acids, thus realizing lysis and binding in one step. The small amount of impurities adsorbed by the magnetic beads is removed by washing with the washing solution. The magnetic beads release the adsorbed nucleic acids under the action of the elution solution, i.e., high quality free (ctDNA, cfDNA, ccfDNA) is obtained. The free DNA extracted by this product does not require toxic reagents such as phenol and chloroform, and the free DNA obtained can be used directly in downstream experiments.

【Product advantages】

1、The extraction effect is good, especially for small fragments and trace DNA, can achieve a high recovery rate.

2、No need to add proteinase K in the extraction process, can be transported and stored at room temperature.

3、Cleavage and binding in one step, no need to pause in the middle to add other reagents.

4、strong versatility, can be used with different manufacturers of automated nucleic acid extraction equipment.

5、can adjust the experiment according to customer needs, to meet the extraction of large volume samples.

6、Good multi-sample compatibility, can be applied to a variety of sample types, such as blood, serum, plasma, saliva, body fluids and so on.

【Product image】

【Experimental cases】

Case 1: A 100bp exogenous nucleic acid fragment was added to serum for simulation, and samples were extracted and subjected to qPCR.

Result analysis：It shows that the reagent system extracts nucleic acids with good purity and reproducibility.

The electrophoresis results were as follows, sample starting volume: 200 μL, 80 μL elution, 6 μL top sample electrophoresis, agarose gel concentration of 1%, 120 V, electrophoresis for 30 min.

Result analysis：As can be seen from the above figure, the experimental results are reproducible and can meet the experimental requirements of downstream qPCR, ddPCR and high-throughput sequencing.

【Application area】

1、Tumor diagnosis and surveillance

It is used to detect circulating tumor DNA (ctDNA), which can analyze tumor gene mutations (e. g., EGFR, KRAS), copy number variation and epigenetic modification, and assist early cancer screening, efficacy evaluation and recurrence monitoring. For example, targeted therapy responsiveness was assessed by dynamic changes in ctDNA.

Non-invasive prenatal testing (NIPT)

Fetal free DNA is extracted from maternal plasma and combined with high-throughput sequencing technology to screen for chromosomal aneuploidy abnormalities such as Down syndrome to avoid the risk of traditional invasive testing.

Pathogen infection detection

Suitable for nucleic acid testing of viruses (e. g. HBV, HPV), bacterial or fungal infectious diseases, especially for the rapid capture of pathogen free DNA in the plasma of sepsis or immunosuppressed patients to improve diagnostic sensitivity.

Genetic analysis

DNA extraction for microdegradation samples, combined with gene mutation detection technology, can analyze the carrier status of genetic diseases.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.