Professional Manufacturer of Biomagnetic Beads
PRODUCT PARAMETERS
- Product No.: 231019M
- Extraction Methods: magnetic bead method
- Sepc: Bottled 50T/box, 100T/box
Description
(Magnetic Bead Method) (Sample Volume 300-500 μL)
【Product Introduction】
This product can be used for the extraction of 300-500 μL of serum/plasma free DNA. The lysis solution not only plays the role of lysing serum/plasma samples, but also provides an environment for the magnetic beads to selectively adsorb nucleic acids, thus realizing lysis and binding in one step. The small amount of impurities adsorbed by the magnetic beads is removed by washing with washing solution. The magnetic beads release the adsorbed nucleic acids under the action of the elution solution, thus obtaining high-quality free DNA. free DNA extracted by this product does not require toxic reagents such as phenol and chloroform, and can be used directly in downstream experiments.
【Product advantages】
1、Good extraction effect, especially for small fragments and trace DNA, can achieve a high recovery rate
2、Cleavage and binding in one step, can get the ideal free DNA in half an hour
3、Strong versatility, can be used with different manufacturers of automated nucleic acid extraction equipment
4、According to customer needs to adjust the experiment, meet the 300-500 mL large volume of sample extraction
5、High flexibility, can be applied to a variety of sample types, such as serum, plasma, cerebrospinal fluid, urine, body fluids and so on. The size and concentration of magnetic beads in the reagent can be adjusted to achieve better nucleic acid extraction for different samples
6、Safe and non-toxic, does not contain phenol and chloroform and other toxic reagents
7、Flexible adjustment of elution volume in the range of 50 μL-100 μL to concentrate circulating DNA
【Product image】
【Experimental cases】
Case 1:
The 100bp exogenous nucleic acid fragment was added to serum for simulation, and samples were extracted and subjected to qPCR.
Result analysis: It indicates that the purity of nucleic acid extracted by the reagent system is good and the reproducibility is good.
The electrophoresis results were as follows, sample starting volume: 200 μL, 80 μL elution, 6 μL top sample electrophoresis, agarose gel concentration of 1%, 120 V, electrophoresis for 30 min.
Result analysis:As can be seen from the above figure, the experimental results are reproducible and meet the requirements of downstream experiments such as qPCR, ddPCR and high-throughput sequencing.
【Application area】
The extracted and purified nucleic acids of this kit can be used in different analysis platforms, such as qPCR, ddPCR and high-throughput sequencing, to meet the needs of human genetic disease screening in early pregnancy and precise medication guidance for tumors.
Supplier Introduction
Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.
We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.
Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.
FAQs
We have magnetic beads in silica, magnetic beads in carboxylic acid, and various kits for nucleic acid extraction by magnetic bead method (samples can be extracted from whole blood, serum plasma free, plasmid, etc.), and the packing specifications of magnetic beads are 2ml, 10ml, 50ml, 500ml, 1000ml, etc. The packing specifications of the kits are: bottles are 50T/box, 100T/box, 200T/box, and the pre-filled kits are 32T/box, 48T/box, 64T/box, 96T/box, and 48T/box. We have 32T/box, 48T/box, 64T/box, 96T/box, and so on.
Land transportation, sea transportation, air transportation, you can choose according to your requirements.
Of course, we provide customized service and also support OEM\ODM.
First, confirm the demand, then sign the contract; after receiving the payment, we will ship the goods within the stipulated or mutually agreed delivery period.
Usually, we will send out the goods within 15 working days after receiving the payment; customized products and large quantities of orders (magnetic beads ≥ 10L, nucleic acid extraction reagents ≥ 300000T) need to be determined by both sides to communicate with the delivery period.