Lnjnbio Magnetic Beads for Polysaccharides and Polyphenols Plant DNA Extraction Kit 160863

(Magnetic Bead Method)

【Product Introduction】

For various polysaccharide and polyphenol plant samples, the nucleic acid is released under the action of lysis solution, the nucleic acid is separated from other impurities in the sample through the adsorption of magnetic beads, and then rinsed by washing solution to remove proteins, polysaccharides, inorganic salt ions, etc., and finally, after elution, the purified nucleic acid is obtained, and the nucleic acid product obtained can be used in experiments such as PCR amplification, nucleic acid detection and analysis, molecular hybridization, gene mutation detection, and sequencing.

【Product advantages】

1, High purity and yield of the extracted product, and for samples with high content of polysaccharides and polyphenols, it also achieves good extraction results;

2, Versatile and suitable for genomic DNA extraction from various polysaccharide and polyphenol plant samples；

3,Safe to use, no need for liquid nitrogen grinding, the product does not contain phenol, chloroform and other harmful reagents.

【Product image】

 

【Experimental case】

Case 1: Dried leaves of different polysaccharide polyphenol plants were used to extract genomic DNA using our polysaccharide polyphenol plant genomic DNA extraction reagent, and the results were as follows:

No. number	Sample	Sample intake（mg）	A260/A280	A260/A230	Conc.(ng/µL)	Yield（µg）
1	Dandelion dried leaves	5 mg	1.767	1.527	264.8	26.48
2			1.779	1.488	188.15	18.82
Avg			1.773	1.5075	226.475	22.65
3	Perilla dried leaves	5 mg	1.85	1.807	267.15	26.72
4			1.84	1.729	266.6	26.66
Avg			1.845	1.768	266.875	26.69
5	Mint dried leaves	5 mg	1.784	1.51	173.8	17.38

Peak pattern diagram:

Dandelion dried leaves:

Perilla dried leaves:

Mint dried leaves:

The electropherogram is shown below:

M:Marker（2K，1K，750bp，500bp，250bp，100bp）;

1-2：5mg Dandelion dried leaves

3-4：5mg Perilla dried leaves

5：5mg Mint dried leaves

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

① The values of A260/A280 were in the range of 1.75~1.85, and the values of A260/A230 were above 1.4, and the peak shapes were complete, which proved that the extracted products were of high purity.

② The nucleic acid extraction yields of different plant leaf samples were above 15 µg.

The results of agarose gel electrophoresis showed that:

The samples of different plant leaves have bright bands and good integrity.

In conclusion, the extracted products of different polysaccharides and polyphenols from dried plant leaves were of high purity, high yield and complete electrophoretic bands.

Case 2:Fresh leaves of different polysaccharide polyphenol plants were used to extract genomic DNA using our polysaccharide polyphenol plant genomic DNA extraction reagent, and the results were as follows:

No.	Sample	Sample intake（mg）	A260/A280	A260/A230	Conc.(ng/µL)	Yield（µg）
1	Dandelion fresh leaves	15mg	1.84	1.698	44.4	4.44
2	Perilla fresh leaves	15mg	1.745	1.379	110.1	11.01
3	Ospecios fresh leaves	10mg	1.755	1.463	55.7	5.57
4	Dichondra fresh leaves	15mg	1.838	1.661	64.65	6.47
5	Desmodium fresh leaves	15mg	1.843	1.777	89.85	8.99
6	Alternanthera philoxeroides fresh leaves	15mg	1.855	1.79	39.65	3.97
7	Plantain fresh leaves	15mg	1.872	1.969	104.2	10.42

Peak pattern diagram:

Dandelion fresh leaves:

Perilla fresh leaves

Ospecios fresh leaves

Dichondra fresh leaves

Desmodium fresh leaves

Alternanthera philoxeroides Fresh leaves

Plantain fresh leaves

The electropherogram is shown below:

M:Marker（2K，1K，750bp，500bp，250bp，100bp）;

1：15mg Dandelion fresh leaves

2：15mg Perilla fresh leaves

3：10mg Ospecios fresh leaves

4：15mg Dichondra fresh leaves

5：15mg Desmodium fresh leaves

6：15mg Alternanthera philoxeroides fresh leaves

7：15mg Plantain fresh leaves

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

① For the samples of different polysaccharide and polyphenol plants, the reagent can stably extract nucleic acids with excellent yield and purity, which proves the versatility of the reagent.

② The values of A260/A280 were in the range of 1.75~1.9, and the values of A260/A230 were above 1.4, and the peak shapes were complete, which proved that the extracted products were of high purity.

③The nucleic acid extraction yield of plant leaf samples of different polysaccharide polyphenol plants was above 4 µg.

The results of agarose gel electrophoresis showed that:

①All the samples of different plant leaves have bright DNA bands with good integrity.

In conclusion, the fresh leaf samples of different polysaccharide polyphenol plants were extracted with high purity, high yield and complete electrophoretic bands.

【Application area】

The extracted and purified DNA of this kit can be directly used in biological experiments such as PCR, Real-time PCR, enzyme digestion, SNP genotyping, Next-generation sequencin, library construction, molecular breeding and so on.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.