Lnjnbio Magnetic Beads for Plasmid DNA Rapid Extraction Kit 160866

(Magnetic Bead Method)

【Product Introduction】

This kit uses alkali lysis method to extract plasmid DNA from cultured bacteria, using the unique solution formula only requires several simple cleaning to remove endotoxin, protein, polysaccharide and other impurities, to obtain high quality plasmid DNA. The extraction of purified plasmid DNA from this product caused little damage to the plasmid, high extraction concentration and high supercoiled ratio. The extracted plasmid DNA can be used in application scenarios including enzyme digestion, PCR, sequencing, ligation and transformation.

【Product advantages】

1, The core raw materials are produced by ourselves, product quality and yield are guaranteed.

2, It can effectively remove endotoxin and improve transfection efficiency.

3, Simple and quick operation with no need for repeated centrifugation.

4, Safe and non-toxic: no need for organic reagents such as phenol/chloroform.

5, High purity of extracted products, high yield, high ratio of super-helix.

6, It can be applied to automation and high throughput operation.

【Product image】

【Experimental cases】

Case 1: Low-copy plasmid was cultured in 2 mL and collected by centrifugation.

The extraction results are as follows:

Number	A260/A280	A260/A230	Conc.(ng/μL)
1	1.987	1.889	293.35
2	1.987	1.889	236.25
3	1.993	1.900	202.90
4	1.976	1.904	259.70
CV	0.3%	0.4%

The electropherogram is shown below:

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay test showed that:：

① CV values were below 1%, demonstrating high reagent extraction stability.

② A260 / A280 range from 1.976 to 1.993, and the values of A260 / A230 range from 1.889 to 1.904. The peak shape is complete and the basic coincidence shows the high purity of the extraction product.

③ 2 mL culture solution can reach more than 20 µ g.

The results of the agarose gel electrophoresis then showed that:

① Four samples were selected, all with bright bands and good integrity.

In conclusion, the plasmid DNA rapid extraction reagent was extracted from four bacterial liquid samples, the extraction product had high purity, high yield, complete electrophoretic strip, and the reagent and the instrument were stable.

Case 2: Low-copy plasmid was cultured in 10 mL and collected by centrifugation. The extraction results are as follows:

Number	A260/A280	A260/A230	Conc.(ng/μL)
1	1.997	2.139	1538.35

The electropherogram is shown below:

Result analysis:

The test results of the ultra-trace nucleic acid tester show that:

① A260 / A280 = 1.997 and A260 / A230 = 2.139, demonstrating the high purity of the extracted product.

② Plasmid extraction yields of more than 150 µg can be achieved with 10mL of culture broth.

The results of the Ultra-Micro Nucleic Acid Assay test showed that:：

① The extracted product ran electrophoresis with bright bands and good integrity.

In conclusion, 10ml of bacterial solution sample was extracted from plasmid DNA, and the extraction products had high purity, high yield and complete electrophoretic bands.

【Application area】

The plasmid DNA extracted using this kit can be used in various biological experiments, such as enzyme digestion, sequencing, library screening, ligation and transformation, etc.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.