Lnjnbio Magnetic Beads for Fungal DNA Extraction Kit 231018

(Magnetic Bead Method)

【Product Introduction】

Fungal mycelium, sporocarp, spores, and other samples release nucleic acid under the action of lysate. The nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed with washing buffer to remove proteins, polysaccharides, inorganic salts, etc., and finally eluted to obtain purified nucleic acid. The obtained nucleic acid products can be used for PCR, DNA detection and analysis, molecular hybridization, gene mutation detection, and sequencing.

【Product advantages】

1. Core raw materials are produced by ourselves and product quality and output are guaranteed.

2. The extraction products had high purity, high yield and complete electrophoretic bands.

3. Magnetic bead method extraction can realize automatic operation.

4. The process of use is safe, and the product does not contain phenol, chloroform and other harmful reagents.

5. Because of strong versatility, it is suitable for a variety of fungi mycelium, sporocarp, spores and other samples for the extraction of nucleic acids.

【Product image】

【Experimental cases】

Case 1:

For different fungal samples, DNA was extracted with the results as follows:

No.	Sample	Sampe intake (mg)	A260/A280	A260/A230	Conc.(ng/μL)
1	Pleurotus eryngii	20	1.84	1.63	346.6
2			1.82	1.68	344.0
3	Pleurotus ostreatus	10	1.82	1.86	278.6
4			1.87	1.91	242.2
5	White Shimeji	10	1.93	2.22	112.5
6			1.96	2.17	97.5

The electropherogram is as follows:

Result analysis: DNA from various fungal samples extracted with this product showed good purity, high yield and complete bands.

Case 2：

For different fungal samples, nucleic acids were extracted and the results were as follows:

No.	Sample intake	A260/A280	A260/A230	Conc.(ng/ul)	Yield(μg)
1	5 mg Yeast	1.948	2.095	95.8	9.58
2		1.953	2.113	100.1	10.01
Avg		1.9505	2.104	97.95	9.795
3	5mg Aspergillus hyphae	1.863	1.863	176.9	17.69
4		1.862	1.868	174.35	17.435
Avg		1.8625	1.8655	175.625	17.5625
5	20mg Lentinula edodes	1.923	2.142	321.55	32.155
6		1.921	2.139	324.05	32.405
Avg		1.922	2.1405	322.8	32.28
7	30 mg Hypsizygus marmoreus	1.94	1.993	619.35	61.935
8		1.944	1.985	626	62.6
Avg		1.942	1.989	622.675	62.2675
9	30 mg Eryngium campestre	1.972	2.181	141.55	14.155
10		1.968	2.194	141	14.1
Avg		1.97	2.1875	141.275	14.1275

Peak pattern diagram:

Yeast:

Aspergillus hyphae:

Lentinula edodes:

Hypsizygusmarm oreus:

Eryngium campestre:

Total peak diagram:

The electropherogram is shown below:

M:Marker（2,000 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, 100 bp）;

1-2: 5 mg Yeast             3-4: 5mg Aspergillus hyphae        5-6: 20mg Lentinula edodes  

7-8: 30 mg Hypsizygus marmoreus                         9-10: 30 mg Eryngium campestre

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay test showed that:

① The value of  A₂₆₀ / A₂₈₀ is from 1.86 to 1.97, and the value of A₂₆₀ / A₂₃₀ is above 1.86. The complete and basic coincidence of peak shape prove the high purity of the extraction product.

② The nucleic acid extraction yield of different fungal samples was above 9 µg.

The results of the agarose gel electrophoresis showed that:

① Different fungal samples have bright and good integrity bands.

② In conclusion, using automatic nucleic acid extraction and purification instrument to extract different fungal samples, the extraction product has high purity, high yield, complete electrophoretic band. The reagent has good stability when used with the instrument.

【Application area】

The purified nucleic acids extracted from this kit can be used for PCR, qPCR, enzyme digestion, library construction, and Southern-Blot.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.