Lnjnbio Magnetic Beads for Fungal DNA Extraction Kit 231018

(Magnetic Bead Method)

【Product Introduction】

Fungal mycelium, sporocarp, spores, and other samples release nucleic acid under the action of lysate. The nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed with washing buffer to remove proteins, polysaccharides, inorganic salts, etc., and finally eluted to obtain purified nucleic acid. The obtained nucleic acid products can be used for PCR, DNA detection and analysis, molecular hybridization, gene mutation detection, and sequencing.

【Product advantages】

1. Core raw materials are produced by ourselves and product quality and output are guaranteed.

2. The extraction products had high purity, high yield and complete electrophoretic bands.

3. Magnetic bead method extraction can realize automatic operation.

4. The process of use is safe, and the product does not contain phenol, chloroform and other harmful reagents.

5. Because of strong versatiCore raw materials are produced by ourselves and product quality and output are guaranteed.

High purity: There is no need to use toxic organic solvents such as phenol and chloroform during the extraction process. This avoids contamination from impurities and ensures high-purity DNA, which can be directly used for molecular biology experiments such as restriction enzyme digestion, PCR, and sequencing.

Easy operation: The manual operation time is short, and it can be used in conjunction with liquid handling workstations or fully automated nucleic acid extraction instruments to achieve automated operation, making it suitable for high-throughput sample processing.

High safety: The extraction process is safe and environmentally friendly, with no involvement of hazardous reagents, protecting the health of laboratory personnel.

Strong versatility: It is applicable to various fungal samples, including mycelium, fruiting bodies, spores, etc.lity, it is suitable for a variety of fungi mycelium, sporocarp, spores and other samples for the extraction of nucleic acids.

【Product image】

【Experimental cases】

Case 1:

For different fungal samples, DNA was extracted with the results as follows:

No.	Sample	Sampe intake (mg)	A260/A280	A260/A230	Conc.(ng/μL)
1	Pleurotus eryngii	20	1.84	1.63	346.6
2			1.82	1.68	344.0
3	Pleurotus ostreatus	10	1.82	1.86	278.6
4			1.87	1.91	242.2
5	White Shimeji	10	1.93	2.22	112.5
6			1.96	2.17	97.5

The electropherogram is as follows:

Result analysis: DNA from various fungal samples extracted with this product showed good purity, high yield and complete bands.

Case 2：

For different fungal samples, nucleic acids were extracted and the results were as follows:

No.	Sample intake	A260/A280	A260/A230	Conc.(ng/ul)	Yield(μg)
1	5 mg Yeast	1.948	2.095	95.8	9.58
2		1.953	2.113	100.1	10.01
Avg		1.9505	2.104	97.95	9.795
3	5mg Aspergillus hyphae	1.863	1.863	176.9	17.69
4		1.862	1.868	174.35	17.435
Avg		1.8625	1.8655	175.625	17.5625
5	20mg Lentinula edodes	1.923	2.142	321.55	32.155
6		1.921	2.139	324.05	32.405
Avg		1.922	2.1405	322.8	32.28
7	30 mg Hypsizygus marmoreus	1.94	1.993	619.35	61.935
8		1.944	1.985	626	62.6
Avg		1.942	1.989	622.675	62.2675
9	30 mg Eryngium campestre	1.972	2.181	141.55	14.155
10		1.968	2.194	141	14.1
Avg		1.97	2.1875	141.275	14.1275

Peak pattern diagram:

Yeast:

Aspergillus hyphae:

Lentinula edodes:

Hypsizygusmarm oreus:

Eryngium campestre:

Total peak diagram:

The electropherogram is shown below:

M:Marker（2,000 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, 100 bp）;

1-2: 5 mg Yeast             3-4: 5mg Aspergillus hyphae        5-6: 20mg Lentinula edodes  

7-8: 30 mg Hypsizygus marmoreus                         9-10: 30 mg Eryngium campestre

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay test showed that:

① The value of  A₂₆₀ / A₂₈₀ is from 1.86 to 1.97, and the value of A₂₆₀ / A₂₃₀ is above 1.86. The complete and basic coincidence of peak shape prove the high purity of the extraction product.

② The nucleic acid extraction yield of different fungal samples was above 9 µg.

The results of the agarose gel electrophoresis showed that:

① Different fungal samples have bright and good integrity bands.

② In conclusion, using automatic nucleic acid extraction and purification instrument to extract different fungal samples, the extraction product has high purity, high yield, complete electrophoretic band. The reagent has good stability when used with the instrument.

【Application area】

Molecular Biology Research

The extracted fungal DNA can be used in various molecular biology experiments, including genome sequencing, gene cloning, PCR amplification, restriction enzyme digestion, and library construction. These experiments help researchers gain a deep understanding of the structure, function, and evolutionary relationships of fungal genomes.

• Microbial Identification and Classification

In the field of microbiology, the reagent can be used to rapidly extract fungal DNA for the identification and classification of different fungal species. Through gene sequencing or PCR technology, the types of fungi can be accurately identified, especially for studies on microbial diversity in complex environmental samples.

• Clinical Diagnosis

In clinical medicine, fungal DNA extraction reagents can be used to detect fungal infectious diseases. By extracting fungal DNA from patient samples and combining it with PCR or sequencing technologies, fungal infections can be diagnosed quickly and accurately, providing a basis for clinical treatment.

• Food Safety Testing

In the field of food safety, the reagent can be used to detect fungal contamination in food, such as molds and yeasts. By extracting fungal DNA from food samples, rapid detection of potential pathogenic fungi can be achieved, ensuring food safety.

• Environmental Microbial Detection

In environmental science, fungal DNA extraction reagents can be used to analyze the diversity of fungi in environmental samples. For example, extracting fungal DNA from soil and water samples helps study the microbial composition and function of ecosystems.

• Forensic Identification

In the field of forensic science, the reagent can be used to extract fungal DNA from biological samples for microbial evidence analysis in cases. For example, detecting specific fungi in environmental samples can provide supplementary evidence for investigations.

• Bioproducts and Cell Culture

In the fields of biotechnology and pharmaceuticals, fungal DNA extraction reagents can be used to detect fungal contamination in bioproducts (such as vaccines and cell therapy products), ensuring product quality and safety.

• High-Throughput Automation Applications

The magnetic bead-based extraction reagent has good compatibility with automation and can be used in combination with automated nucleic acid extraction instruments to achieve high-throughput sample processing. This is particularly advantageous for large-scale sample testing, improving efficiency and reducing human error.

• Gene Function Research

The extracted fungal DNA can be used for gene function studies, such as investigating the functions of fungal genes through techniques like gene knockout and overexpression.

1. Molecular Diagnostics and Nucleic Acid Testing

In the field of molecular diagnostics, the reagent can be used to extract fungal DNA from samples and, combined with real-time quantitative PCR (qPCR) or digital PCR (dPCR) technologies, achieve highly sensitive detection of fungi.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.