Lnjnbio Magnetic Beads for FFPE Tissue DNA Extraction Kit 221006

（Magnetic Beads Method）

【Product Introduction】

The paraffin-embedded tissue DNA extraction reagent uses an environmentally friendly deparaffinization method to remove paraffin, and special lysis conditions are applied to release the DNA in the tissue section, overcoming the inhibition effect caused by formalin cross-linking. The reagent uses DNA-binding magnetic beads and a unique buffer system to purify high-quality DNA while maximizing the removal of proteins, inorganic salts and other organic impurities. The obtained DNA has good integrity, high purity, stable and reliable quality, and is suitable for downstream experiments such as PCR, qPCR, SNP gene analysis, and sequencing.

【Product advantages】

1. Safe and non-toxic: no need for xylene dewaxing, no need for toxic organic reagents such as phenol/chloroform

2. Excellent applicability: suitable for paraffin sections of various tissues

3. High purity: effectively remove PCR inhibitors such as proteins and inorganic salts, which is convenient for downstream experiments.

4. This kit can be used for manual extraction, and can also be integrated with a variety of high-throughput extractors.

【Product image】

 

【Experimental case】

Case1：

The DNA of 10 cases of paraffin-embedded tissues (5 μm, 6 sections) of human gastric mucosa was extracted by using our company’s paraffin-embedded tissue DNA extraction kit, and then qPCR was performed with human GAPDH housekeeper gene as the target gene, and whether the extracted product affected qPCR amplification.

Result analysis: The results showed that the amplification curve was S-shaped, and there was no obvious inhibition. The results show that the DNA extracted by this reagent is of excellent quality and can be directly used in downstream PCR and other follow-up experiments.

Case2：

The DNA of paraffin-embedded tissue DNA extraction reagent was used to extract the paraffin-embedded tissue (20 μm, 1 sections) of pig lung, canine pancreas, and chicken lung, and the extracted products were tested by ultra-micro-ultraviolet-visible spectrophotometer, and the results were as follows:

No.	Sample	A260/A280	A260/A230	Conc.(ng/μL)	Yield（μg）
1	Porcine lung embedded tissue sections	1.75	2.104	297.1	29.71
2		1.76	2.115	280.1	28.01
3	Canine pancreatic embedded tissue sections	1.754	2.089	158.4	15.84
4		1.709	2.083	195.55	19.555
5	Chicken lung embedded tissue sections	1.616	1.946	114.8	11.48
6		1.606	1.826	108.55	10.855

Results: The results showed that the reagent could stably extract DNA with good purity and high yield for different types of embedded tissues, which could be directly used in downstream PCR and other follow-up experiments.

【Application area】

The purified DNA extracted from this kit can be directly used in downstream molecular biology experiments such as PCR, qPCR, dPCR, NGS, SNP gene analysis, STR gene identification, pathological molecular research, etc., and can be applied to identification, disease diagnosis, personalized medicine and other fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.