Endotoxin-free Plasmid DNA Extraction Kit for Small-volume Samples 241001S

(Magnetic Bead Method)

【Product Introduction】

This product uses alkali lysis method to extract plasmid DNA from cultured bacteria, using the unique solution formula only needs a few simple cleaning to remove endotoxin, protein, polysaccharide and other impurities, to obtain high quality plasmid DNA. The extraction of purified plasmid DNA from this product caused little damage to plasmid, high extraction concentration and high supercoiled ratio. The plasmid DNA extracted by this product is suitable for downstream applications that are sensitive to endotoxins, such as transfection experiments, gene therapy, and in vitro transcription.

【Product advantages】

1. Core Materials Self-Produced: The core raw materials are manufactured in-house, ensuring both the quality and yield of the product.

2. High-Purity Endotoxin-Free: The extracted plasmid DNA has extremely low endotoxin content, making it suitable for cell transfection and in vivo experiments.

3. Efficient Extraction: The magnetic bead method efficiently binds plasmid DNA, resulting in high extraction efficiency.

4. Fast and Convenient: The extraction process can be completed within 1 hour and is compatible with fully automated nucleic acid extraction and purification instruments, enabling automated and high-throughput operations.

5. Broad Applicability: Suitable for the extraction of both high-copy and low-copy plasmids.

【Product image】

【Experimental cases】

Case 1: 

(1) The sample is DH 5α E. coli (containing PHY300PLK-egls gene plasmid, base pair about 6000 bp, low copy plasmid), bacterial solution OD₆₀₀ = 0.11. After extraction was determined by Quawell Q5000 trace ultraviolet visible spectrophotometer, the results are as follows:

Number	Bacterial Solution Volume(mL)	A260/A280	A260/A230	Conc.(ng/μL)	Elution Volume (µL)	Yield(ng)
1	5	1.786	1.953	259.65	100	25.97
2		1.792	1.975	265.5		26.55
Average		1.789	1.964	262.58		26.26

The peak patterns are as follows:

The electrophoresis results are as follows:

Note: the original concentration of 6 μL on the sample electrophoresis, agarose gel concentration of 1%, 120 V electrophoresis.

(2) Results analysis:

Quawell Q5000 The results of microultraviolet visible spectrophotometer show that:

① The value of  A₂₆₀ / A₂₈₀ is around 1.7 and A₂₆₀ / A₂₃₀ is around 1.9, demonstrating the high purity of the extracted product.

② Under OD₆₀₀ = 0.11, the yield of 5 mL of culture solution can reach about 26 ng.

The results of the agarose gel electrophoresis then showed that:

The bands are bright and complete.

In conclusion, 5 mL of endofree DNA was extracted from plasmid, producing the products with high purity, high yield and complete electrophoretic bands.

Case 2: 

(1) The sample is DH 5α E. coli (containing PHY300PLK-egls gene plasmid, base pair about 6000 bp, low copy plasmid), bacterial solution OD₆₀₀ = 0.239. Plasmid was extracted by LJbio32H automated nucleic acid extraction instrument (combined with the instrument operation, the amplitude was level 3). After extraction was determined by Quawell Q5000 trace ultraviolet visible spectrophotometer, the results are as follows:

Number	Bacterial Solution Volume(mL)	A260/A280	A260/A230	Conc.(ng/μL)	Elution Volume (µL)	Yield(ng)
1	4	1.903	2.39	1124.3	100	0.11243
2		1.902	2.387	1130.5		0.11305
Average		1.9025	2.3885	1127.4		0.11274

The peak patterns are as follows:

The electrophoresis results are as follows::

Note: the original concentration of 6 μL on the sample electrophoresis, agarose gel concentration of 1%, 120 V electrophoresis.

(2) Results analysis:

Quawell Q5000 The results of microultraviolet visible spectrophotometer show that:

① The value of  A₂₆₀ / A₂₈₀ is around 1.9 and A₂₆₀ / A₂₃₀ is around 2.3, demonstrating the high purity of the extracted product.

② Under OD₆₀₀ = 0.239, the yield of 4 mL of culture solution can reach about 0.11 mg.

The results of the agarose gel electrophoresis then showed that:

The bands are bright and complete.

In conclusion, 4 mL of endofree DNA was extracted from plasmid, producing the products with high purity, high yield and complete electrophoretic bands.

【Application area】

The plasmid DNA extracted using this kit can be applied in the following research fields:

1. Transfection and Cell Culture: The extracted plasmid DNA has low endotoxin levels, making it suitable for applications such as transfection of eukaryotic cells and in vitro transcription.

2. Molecular Biology Experiments: Suitable for routine operations such as restriction enzyme digestion, PCR, sequencing, ligation, and transformation.

3. High-Throughput Screening: Supports automated extraction, ideal for the construction and screening of large-scale plasmid libraries.

4. Clinical and Research Applications: Suitable for applications with extremely high requirements for plasmid purity, such as gene therapy and vaccine development.
【Supplier Introduction】

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.