Endotoxin-free Plasmid DNA Extraction Kit for Large-volume Samples 241001L

(Magnetic Bead Method)

【Product Introduction】

This product uses alkali lysis method to extract plasmid DNA from cultured bacteria, using the unique solution formula only needs a few simple cleaning to remove endotoxin, protein, polysaccharide and other impurities, to obtain high quality plasmid DNA. The extraction and purification of plasmid DNA using this product cause minimal damage to the plasmid, resulting in high concentration and a high proportion of supercoiled DNA. The plasmid DNA extracted by this product is suitable for downstream applications that are sensitive to endotoxins, such as transfection experiments, gene therapy, and in vitro transcription.

【Product advantages】

1.Core Materials Self-Produced: The core raw materials are manufactured in-house, ensuring both the quality and yield of the product.

2. High-Purity Endotoxin-Free: The extracted plasmid DNA has extremely low endotoxin content, making it suitable for cell transfection and in vivo experiments.
3. Efficient Extraction: The magnetic bead method efficiently binds plasmid DNA, achieving high extraction efficiency and is suitable for large-scale extraction (up to 1 mg or more).
4. Fast and Convenient: The extraction process can be completed within 1 hour and can be automated using a fully automatic nucleic acid extraction and purification instrument, enabling high-throughput operations.
5. Broad Applicability: Suitable for the extraction of both high-copy and low-copy plasmids.
6. High Yield and Purity: From 100 mL of bacterial culture (low-copy plasmid), a yield of up to 1.17 mg can be achieved. The OD260/OD280 ratio is typically between 1.7 and 2.0, with a high proportion of supercoiled DNA.
7. Downstream Compatibility: The extracted plasmid DNA is ready for downstream applications such as transfection, sequencing, PCR, and restriction enzyme digestion.
8. Simple, Fast, and Safe: The operation is straightforward and rapid, without the need for toxic organic reagents such as phenol or chloroform.

【Product image】

【Experimental cases】

Case: 

The sample is DH 5α E. coli (containing PHY300PLK-egls gene plasmid, base pair about 6000 bp, low copy plasmid), bacterial solution OD₆₀₀ = 0.103. Plasmid was extracted by LJbio32H automated nucleic acid extraction instrument (combined with the instrument operation, the amplitude was level 3). After extraction was determined by Quawell Q5000 trace ultraviolet visible spectrophotometer, the results are as follows:

Number	Bacterial Solution Volume(mL)	A260/A280	A260/A230	Conc.(ng/μL)	Elution Volume (mL)	Yield(mg)
1	100	1.756	1.99	464.95	1	1.162
2		1.757	1.996	468.15		1.170
Average		1.756	1.993	466.55		1.166
3	200	1.819	2.053	840.35		2.100
4		1.818	2.059	830.55		2.076
Average		1.819	2.056	835.45		2.089

The peak patterns are as follows:

The electrophoresis results are as follows::

Note: the original concentration of 6 μL on the sample electrophoresis, agarose gel concentration of 1%, 120 V electrophoresis.

(2) Results analysis:

Quawell Q5000 The results of microultraviolet visible spectrophotometer show that:

① The value of  A₂₆₀ / A₂₈₀ is 1.75-1.81 and A₂₆₀ / A₂₃₀ is 1.9-2.0, demonstrating the high purity of the extracted product.

② Under OD₆₀₀ = 0.103, the yield of 100 mL/200mL of culture solution can reach about 1.166 mg/2.1 mg, respectively.

The results of the agarose gel electrophoresis then showed that:

The bands are bright and complete.

In conclusion, 100 mL and 200 mL of endofree DNA was extracted from plasmid, producing the products with high purity, high yield and complete electrophoretic bands.

【Application area】

The plasmid DNA extracted using this kit can be applied in the following research fields:

1. Transfection and Cell Culture: The extracted plasmid DNA has low endotoxin levels, making it suitable for applications such as transfection of eukaryotic cells and in vitro transcription.

2.  Molecular Biology Experiments: Suitable for routine operations such as restriction enzyme digestion, PCR, sequencing, ligation, and transformation.

3. High-Throughput Screening: Supports automated extraction, ideal for the construction and screening of large-scale plasmid libraries.

4. Clinical and Research Applications: Suitable for applications with extremely high requirements for plasmid purity, such as gene therapy and vaccine development.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.