Endotoxin-free Plasmid DNA Extraction Kit for Large-volume Samples 241001L

(Magnetic Bead Method)

【Product Introduction】

This product uses alkali lysis method to extract plasmid DNA from cultured bacteria, using the unique solution formula only needs a few simple cleaning to remove endotoxin, protein, polysaccharide and other impurities, to obtain high quality plasmid DNA. The extraction of purified plasmid DNA from this product caused little damage to plasmid, high extraction concentration and high supercoiled ratio. Plasmid DNA prepared by EndoFree Mini Plasmid Kit is suitable for a variety of routine applications including restriction enzyme digestion, PCR, sequencing, ligation, and transformation.

【Product advantages】

1. Our own production of core raw materials, product quality and output are guaranteed.

2. It can effectively remove the endotoxin and improve transfection efficiency.

3. Extraction plasmid DNA purity is high. OD₂₆₀ / OD₂₈₀ is usually around 17-2.0. The yield of 100 mL bacterial solution (low copy plasmid) can reach 1.17 mg. Supercoil ratio is high.

4. Simple operation, fast, safe, no need for phenol / chloroform and other toxic organic reagents.

• It can cooperate with the automatic nucleic acid extraction and purification instrument to realize the automatic and high-throughput operation.

【Product image】

【Experimental cases】

Case: 

The sample is DH 5α E. coli (containing PHY300PLK-egls gene plasmid, base pair about 6000 bp, low copy plasmid), bacterial solution OD₆₀₀ = 0.103. Plasmid was extracted by LJbio32H automated nucleic acid extraction instrument (combined with the instrument operation, the amplitude was level 3). After extraction was determined by Quawell Q5000 trace ultraviolet visible spectrophotometer, the results are as follows:

Number	Bacterial Solution Volume(mL)	A260/A280	A260/A230	Conc.(ng/μL)	Elution Volume (mL)	Yield(mg)
1	100	1.756	1.99	464.95	1	1.162
2		1.757	1.996	468.15		1.170
Average		1.756	1.993	466.55		1.166
3	200	1.819	2.053	840.35		2.100
4		1.818	2.059	830.55		2.076
Average		1.819	2.056	835.45		2.089

The peak patterns are as follows:

The electrophoresis results are as follows::

Note: the original concentration of 6 μL on the sample electrophoresis, agarose gel concentration of 1%, 120 V electrophoresis.

(2) Results analysis:

Quawell Q5000 The results of microultraviolet visible spectrophotometer show that:

① The value of  A₂₆₀ / A₂₈₀ is 1.75-1.81 and A₂₆₀ / A₂₃₀ is 1.9-2.0, demonstrating the high purity of the extracted product.

② Under OD₆₀₀ = 0.103, the yield of 100 mL/200mL of culture solution can reach about 1.166 mg/2.1 mg, respectively.

The results of the agarose gel electrophoresis then showed that:

The bands are bright and complete.

In conclusion, 100 mL and 200 mL of endofree DNA was extracted from plasmid, producing the products with high purity, high yield and complete electrophoretic bands.

【Application area】

The plasmid DNA extracted using this kit can be used in various biological experiments, such as enzyme digestion, sequencing, library screening, ligation and transformation, etc.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.