Lnjnbio Magnetic Beads for Bacteria Genomic DNA Extraction Kit 160886

PRODUCT PARAMETERS

  • Product No.: 160886
  • Extraction Methods: magnetic bead method
  • Sepc: Bottled 50T/box, 100T/box, 200T/box
Description
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Description

(Magnetic Bead Method)

【Product Introduction】

Gram-negative bacteria or positive bacteria samples, under the action of lysis buffer, release the nucleic acid, and specific adsorption of nano magnetic beads separates the nucleic acid from other impurities in the sample. After washing buffer, remove proteins, inorganic salt ions, etc., and finally after elution,the purified nucleic acid is obtained. The nucleic acid products obtained can be used for PCR amplification, nucleic acid detection and analysis, molecular hybridization, gene mutation detection and sequencing, and other experiments.

【Product advantages】

  1. 1. After breaking the bacteria’s wall, without centrifugation, directly adsorb the target fragment with magnetic bead extraction.
  2. The extracted products showed high purity, yield, and complete electrophoretic bands.
  3. Magnetic bead method extraction can realize automatic operation.
  4. The use process is safe, without liquid nitrogen grinding, the product does not contain phenol, chloroform, and other harmful reagents.

【Product image】

Bacteria Genomic DNA Extraction Kit

【Experimental cases】

Case 1: E. coli was grown overnight for 15 h, the bacteria of 1mL were collected by centrifugation, and genomic DNA was extracted. The results were as follows:

NumberA260/A280A260/A230Conc.(ng/μL)
11.852.03121.8
21.821.99206.7
31.801.94153.2
41.821.90181.6
51.841.91177.2
61.801.86185.9
71.832.24155.3
81.842.34148.1

Agarose gel electrophoresis:

M:Marker(2,000 bp, 1,000 bp, 750 bp, 500 bp, 250 bp, 100 bp);

Result analysis: In conclusion, the nucleic acid extraction yield of E.coli has high purity, high yield, and complete electrophoretic band. 

【Application area】

The purified nucleic acids extracted from this kit can be used for PCR, qPCR, enzyme digestion, library construction, and Southern Blot.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence, and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.

FAQs

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