(Magnetic Bead Method)

【Product Introduction】

This product is suitable for animal muscle, liver, spleen, and other animal tissue and cultured cell samples, the basic step is under the lysate release nucleic acid, magnetic beads specific adsorption DNA, remove protein, polysaccharide, inorganic salt ions impurities, finally after low salt elution to high purity nucleic acid, nucleic acid products can be used for PCR, nucleic acid detection analysis, molecular hybridization, gene mutation detection and sequencing experiments.

【Product advantages】

1、Extraction by magnetic bead method and suitable for large-scale sample processing without repeated centrifugation.

2、High purity of extracted products, high yield, complete electrophoretic bands

3、Versatile and suitable for extracting genomic DNA from a variety of tissue samples from different species of animals

4、Safe to use, no need for liquid nitrogen grinding, the product does not contain phenol, chloroform, and other harmful reagents

5、Cost-effective, the extraction effect is comparable to that of similar products, but at a cheaper price

【Product image】

【Experimental case】

Case: Genomic DNA was extracted from different tissue samples of different animals with the following results:

Sample number	Sample	A260/A280	A260/A230	Conc.(ng/ul)	Yield（μg）	Coefficient of variation
1	20mg Shrimp	1.886	2.037	113.1	11.31
2		1.897	2.058	113.4	11.34
Average		1.8915	2.0475	113.25	11.325	0.13%
3	20mg Chicken meat	1.889	2.06	139.5	13.95
4		1.894	2.064	141.05	14.105
Average		1.8915	2.062	140.275	14.0275	0.55%
5	30mg Pork	1.859	2.057	139.7	13.97
6		1.866	2.054	140.5	14.05
Average		1.8625	2.0555	140.1	14.01	0.29%
7	30mg Goat meat	1.851	1.935	137.8	13.78
8		1.85	1.946	148.15	14.815
Average		1.8505	1.9405	142.975	14.2975	3.62%
9	30mg Beef	1.847	1.935	89.9	8.99
10		1.85	1.943	90.7	9.07
Average		1.8485	1.939	90.3	9.03	0.44%
11	30mg Fish	1.873	2.097	237	23.7
12		1.875	2.083	233.2	23.32
Average		1.874	2.09	235.1	23.51	0.81%
13	3mg Chicken heart	1.836	2.116	187.3	18.73
14		1.837	2.107	183.65	18.365
Average		1.8365	2.1115	185.475	18.5475	0.98%
15	3mg Chicken liver	1.864	1.986	130.5	13.05
16		1.861	1.991	138.6	13.86
Average		1.8625	1.9885	134.55	13.455	3.01%
17	3mg Fish liver	1.768	2.285	371.45	37.145
18		1.769	2.29	369.85	36.985
Average		1.7685	2.2875	370.65	37.065	0.22%
19	3mg Fish heart	1.827	2.236	140.4	14.04
20		1.818	2.201	145.7	14.57
Average		1.8225	2.2185	143.05	14.305	1.85%

Plot of the peak pattern of the micronuclei acid assay test:

Shrimp:

Chicken meat:

Pork:

Goat meat:

Beef:

Fish:

Chicken heart

Chicken liver:

Fish liver:

Fish heart:

The electropherogram is shown below:

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

①A260/A280, A260/A230, the coefficient of variation was below 3.62%, which proved the high stability of reagent extraction.

②The values of A260/A280 were in the range of 1.8~1.9, the values of A260/A230 were above 2.0, and the peak shapes were complete and overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields ranged from 8-37 µg or more for different animal tissue samples.

The results of agarose gel electrophoresis showed that:

①Various tissue samples from different animals with bright bands and good integrity.

In conclusion: the extraction of different animal samples using the fully automated nucleic acid extractor showed high purity of the extracted products, high yield, complete electrophoretic bands, and uniform and stable cooperation between the reagents and the instrument.

【Application area】

Scientific research use

1. Genomics research: used to construct genome libraries, conduct genome sequencing and assembly, and reveal the structure and function of animal genomes.

2. Gene expression study: as the template for PCR, qPCR, and other experiments, to study the gene expression level and regulation mechanism.

3. Genetic variation analysis: This technique detects SNPs, insertion deletions, and other genetic variations in animal genomes and provides a basis for genetic breeding and evolutionary research.

4. Molecular marker-assisted breeding: develop molecular markers, assist animal breeding, and improve the efficiency and accuracy of breeding.

Medical use

1. Disease diagnosis: detect genetic variants related to disease to assist clinical diagnosis.

2. Disease research: to study the occurrence mechanism and genetic background of animal diseases, and to provide the scientific basis for disease prevention and treatment.

Industrial use

1. Food safety testing: This is used to detect animal ingredients in food and ensure its authenticity and safety.

Other uses

1. sample preparation for high-throughput sequencing: The extracted high-quality genomic DNA can be used for sample preparation for high-throughput sequencing, providing support for large-scale genomics research.

2. Automatic nucleic acid extraction platform: it can be integrated into the automatic nucleic acid extraction platform to achieve high-throughput, fast, and stable nucleic acid extraction.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence, and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.