How to Ensure the Quality of RNA Extraction from Urine and Other Samples？

Urine and vaginal secretions, semen, tears and other samples have become popular choices in research because of their non-invasiveness and easy access. Therefore, correct pretreatment steps are crucial to extract high-quality RNA from these samples. The following are several pretreatment tips that can help improve the quality of RNA from urine and other samples.

Keeping enough sample volume

The RNA content in urine samples is relatively low, so keeping a larger sample volume is recommended, which can not only improve the recovery rate of RNA, but also increase the overall quality of RNA extraction.

Standardized sample collection

The quality of urine samples is affected by many factors, including collection time, urination frequency, etc. Establishing a standardized collection procedure can help reduce the impact of these variables on sample quality.

Use of protectants

Although urine samples are unlikely to require additional protectants when cryopreserved, in some cases, the use of protectants such as RNAlater can help maintain RNA stability.

Appropriate centrifugation

Centrifugation can help remove precipitates and other impurities in urine, ensuring that they won’t disturb the RNA extraction.

Processing of other samples

Using sperm washing solution to purify sperm can completely remove the contamination from other cells, laying a good foundation for the next step of extracting high-quality sperm RNA. For vaginal secretions, 10 mL of vaginal lavage fluid is usually collected, and the precipitate is stored in 1 mL RNAlater after centrifugation and then stored at -70℃ for later use. The best pretreatment method for tear samples is to put the tear samples into 1.5 mL RNase-free centrifuge tubes, freeze them at -70℃, and then extract RNA when needed. Low-temperature freezing can maximize the quality of RNA.

The above pretreatment strategy can improve the quality of RNA in urine and other samples effectively, providing a solid foundation for subsequent research work.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 which is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid isolation and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, etc. We not only provide products but also can undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.