How to efficiently extract nucleic acids from plants

In modern molecular biology research, plant nucleic acid extraction is the basis of genomics, transcriptomics, functional genomics, and other fields. However, traditional methods often face the interference of secondary metabolites such as polysaccharides and polyphenols, resulting in low purity and unstable yield of DNA/RNA, which makes it difficult to meet the requirements of high-throughput sequencing and accurate quantitative analysis. Especially in universities and scientific research units, the demand for high-quality and standardized plant nucleic acids is particularly urgent. And the magnetic bead method nucleic acid extraction reagents came into being, aiming to solve these challenges and provide reliable support.

I. Product features:

Wide applicability: applicable to a wide range of plant samples, including leaves, roots, seeds, pollen, etc., covering a wide range of fields from model plants to economic crops.

Efficient and stable: Using advanced magnetic bead method technology, it can complete nucleic acid extraction quickly while ensuring the integrity and purity of DNA/RNA. Ideal extraction results can be achieved even for complex samples containing large inhibitors.

Simple and easy to use: Optimized procedures and clear instructions make it easy for beginners to get started. The accompanying automation solution further enhances work efficiency.

Highly compatible: The extracted nucleic acids are suitable for a variety of downstream applications such as PCR amplification, qPCR quantification, sequencing analysis, etc., and can be used directly without additional processing.

Batch consistency: A strict quality control system ensures that the performance of each batch of products is consistent, reducing the experimental errors caused by the differences between batches of reagents.

Second, technical advantages:

Lysis buffer: The specially formulated lysis buffer can effectively destroy the cell structure and release intact nucleic acid molecules while maximizing the removal of polysaccharides, polyphenols, and other impurities.

Magnetic bead selection: Superparamagnetic nano-magnetic beads are selected for their strong binding ability, which improves the recovery rate and purity of nucleic acids.

Anti-pollution design: The core raw materials used undergo strict aseptic treatment and full monitoring is implemented during the production process to ensure that the product does not contain exogenous DNA, RNA, or enzymes to avoid the risk of cross-contamination.

Environmental friendly: no pollution, no use of chloroform, isoamyl alcohol, phenol, and other toxic reagents.

Third, application scenarios:

Gene expression analysis: extract total RNA, support the isolation and purification of mRNA, miRNA, and other small RNA, and provide reliable templates for the construction of gene expression profiles.

Genetic diversity research: helps researchers obtain high-quality genomic DNA from different species or individuals, and supports population genetics, evolutionary biology, and other fields.

Functional genomics: suitable for the application of CRISPR/Cas9 and other gene editing tools, providing pure target DNA for gene knockout, overexpression, and other experiments.

Teaching and training: as a standard, it is used for teaching demonstrations in molecular biology courses to cultivate students’ practical skills.

Magnetic Bead Method Nucleic Acid Extraction Kit has become an indispensable right-hand man for universities and research institutes by virtue of its wide applicability, excellent technical performance, rich application scenarios, and simplified operation process. It not only significantly improves experimental efficiency and data quality, but also brings more convenience and possibilities for teaching and research work.