Lnjnbio Magnetic Beads for Polysaccharides and Polyphenols Plant Total RNA Extraction Kit231001

(Magnetic Bead Method)

【Product Introduction】

This kit uses magnetic beads and buffer system with unique separation effect to extract total RNA from roots, stems, leaves and seeds of polysaccharide and polyphenol plants, and the whole process is easy to operate especially suitable for automated nucleic acid extraction platform. The total RNA extracted is of high yield, high purity, stable and reliable quality, and can be applied to various downstream molecular biology experiments.

【Product advantages】

Magnetic bead-based extraction eliminates the need for repeated centrifugation, ensuring the integrity of the extracted total RNA.

2. The extracted product is of high purity and yield, with complete electrophoresis bands, and can be directly used for various downstream applications such as high-throughput sequencing, RT-PCR, microarray analysis, and Northern Blot.

3. The process is safe to use, without the need for liquid nitrogen grinding, and the product does not contain toxic reagents such as phenol or chloroform.

4. Simple and rapid operation: The entire extraction process is time-efficient and does not involve toxic organic solvents like phenol or chloroform, making it safe and environmentally friendly.

5. Wide sample compatibility: Suitable for a variety of plant samples, including perilla leaves, mint leaves, and dandelion.

6. Automation compatibility: It can be perfectly integrated with multiple automated nucleic acid extraction instruments to achieve high-throughput automated extraction.

【Product image】

【Experimental case】

Case 1:Leaf samples of different polysaccharide polyphenol plants were extracted for total RNA with the following results:

No.	Sample	A260/A280	A260/A230	Conc.(ng/µL)	Yield（µg）
1	10mg Perilla leaves	1.964	1.641	71.92	7.192
2		1.965	1.653	71.92	7.192
Avg		1.9645	1.647	71.92	7.192
3	10mg Mint leaves	1.967	2.124	149.72	14.972
4		1.961	2.158	149.76	14.976
Avg		1.964	2.141	149.74	14.974
5	10mg Dandelion leaves	1.951	2.232	219.16	21.916
6		1.959	2.228	220.4	22.04
Avg		1.955	2.23	219.78	21.978

Peak pattern diagram:

Perilla leaves:

Mint leaves:

Dandelion leaves:

Total peak diagram:

The electropherogram is shown below:

 

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

The results of the ultra-micro nucleic acid assay showed that:

① The values of A260/A280 were in the range of 1.9~2.0, and the values of A260/A230 were above 1.6, and the peak shapes were complete and basically overlapped, which proved that the extracted products were of high purity.

②The yields of nucleic acids extracted from the leaves of different plants were above 7 µg.

The results of agarose gel electrophoresis showed that:

① All the samples of different polysaccharide polyphenol plant leaves have bright bands and good integrity.

In conclusion, the extracted products of different polysaccharide polyphenol plant leaf samples were of high purity, high yield and complete electrophoretic bands.

【Application area】

The RNA extracted by this kit can be applied to the following research fields:

1. Plant Science Research

Plant Gene Expression Studies: It can be used to analyze changes in gene expression at different growth stages or under stress conditions.

Plant Breeding: By extracting and analyzing plant RNA, genes related to desirable traits can be identified, providing theoretical support for plant breeding.

Plant Growth and Development Studies: RNA extracted from different plant tissues (such as roots, stems, leaves, flowers, and fruits) can be used to study the genetic regulatory mechanisms during growth and development.

Stress Biology Research: It can be used to analyze changes in gene expression under adverse conditions such as drought, salinity, and high temperature.

• Gene Expression Analysis

Real-time Quantitative PCR (qRT-PCR): The extracted RNA can be used for quantitative analysis of specific gene expression levels.

RNA Sequencing (RNA-seq): High-quality RNA is essential for transcriptome sequencing and can be used for comprehensive analysis of plant transcriptomes.

Microarray Analysis: The extracted RNA can be used for microarray analysis to study gene expression profiles.

• Molecular Biology Experiments

Northern Blot and Dot Blot: These techniques are used to detect specific RNA bands or dot arrays.

PolyA Selection: It is used for enriching and studying mRNA.

In Vitro Translation: The extracted RNA can be used for in vitro translation experiments to study protein synthesis.

RNase Protection Assay: It is used to analyze RNA stability.

Molecular Cloning: The extracted RNA can be used for constructing cDNA libraries.

• High-Throughput Automated Extraction

Compatibility with Automated Extraction Devices: The magnetic bead-based extraction reagents are suitable for use with automated nucleic acid extraction instruments, allowing for rapid and efficient processing of large sample volumes.

High-Throughput Experiments: It is suitable for large-scale RNA extraction from plant samples, improving experimental efficiency.

• Other Applications

Non-coding RNA Research: The extracted RNA can be used to study the functions of non-coding RNAs such as miRNA and lncRNA.

Protein Synthesis and Modification Studies: By analyzing mRNA modifications (such as m6A methylation), the impact on translation efficiency can be studied.

Environmental Microbiology: It can be used to study the RNA of microbial communities in the rhizosphere of plants.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.