Lnjnbio Magnetic Beads for Whole Blood Genomic DNA Extraction Kit 231006(Magnetic Bead Method)

【Product Introduction】

Whole blood sample under the action of guanidinium salt cells are lysed and release nucleic acid, through the specific adsorption of magnetic beads to separate the nucleic acid from other impurities in the sample, and then rinsed by the Wash Buffer, to remove the protein, residual guanidinium salt, etc., and finally after elution, to get a good purified nucleic acid, suitable for the extraction of genomic DNA from human whole blood, and the nucleic acid product obtained can be used for PCR, nucleic acid detection and analysis, in vitro diagnosis, gene mutation detection and sequencing, etc.

【Product advantages】

1. For difficult to cleave samples (blood clot, etc.) can also realize the cleavage of the combination of one-step, do not need to pause in the middle to join other reagents

2. The extracted genomic DNA has good purity, high yield and complete electrophoretic bands.

3. No need to add proteinase K in the extraction process, and it can be transported and stored at room temperature.

4. High extraction efficiency, the whole extraction process only takes about 15 minutes.

5. The product does not contain phenol, chloroform and other harmful reagents, the use of the process is safe.

6. Strong applicability, can be used with different manufacturers of automated nucleic acid extraction equipment.

【Product image】

【Experimental cases】

Case 1:

Experimental results of the same batch of reagents for extracting fresh blood samples from different healthy individuals:

Sample number	Sample	A260/A280	A260/A230	Conc.(ng/µL)
1	Blood sample No. 1	1.80	1.89	75.7
2	Blood sample No. 2	1.89	1.89	73.9
3	Blood sample No. 3	1.83	1.93	68.2
4	Blood sample No. 4	1.82	1.90	67.9
5	Blood sample No. 5	1.84	1.99	59.1

【The electropherogram is shown below】

Result analysis: The human blood DNA extracted with this kit has good purity, high concentration and complete electrophoretic bands.

Case 2：

Experimental results of different batches of reagents for extraction of the same pig blood sample:

Kit lot	A260/A280	A260/A230	Conc.(ng/µL)
Lot 1	1.87	1.90	157.5
Lot 1	1.87	1.96	137.9
Average	1.87	1.93	147.7
Lot 2	1.88	1.92	132.6
Lot 2	1.88	1.99	140.4
Average	1.88	1.96	136.5
Lot 3	1.89	1.95	141.2
Lot 3	1.89	1.91	141.7
Average	1.89	1.93	141.4
Inter-lot average	1.88	1.94	141.87
Inter-lot CV	0.4%	0.7%	3.2%

Result analysis：Extraction kits have low lot-to-lot variation and good stability.

Case 3：

Experimental results of different batches of reagents for extraction of the same pig blood sample:

Kit lot	A260/A280	A260/A230	Conc.(ng/μL)
Lot 1	1.952	2.061	144.85
	1.96	2.061	116.55
	1.93	2.018	149.3
Average	1.947	2.046	136.9
Lot 2	1.952	1.987	137.45
	1.947	1.983	137.9
	1.945	2.024	148.45
Average	1.948	1.998	141.267
Inter-lot average	1.948	2.022	139.084
Inter-lot CV	0.02%	1.1%	1.5%

Result analysis：Extraction kits have low lot-to-lot variation and good stability.

【Application area】

The extracted and purified nucleic acids of this kit are suitable for various routine experiments such as enzyme digestion, PCR, library construction, Southern hybridization, and microarray hybridization and high-throughput sequencing.

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at sales01@lingjunbio.com.