Swabs Nucleic Acid Extraction Kit 221004

This product is mainly used for the extraction, enrichment and purification steps of nucleic acid in swab wash, virus preservation solution, saliva, semen, urine, lymph fluid and many other body fluid samples and animal tissue cell homogenate. The sample is lysed and released nucleic acid under the action of guanidine salt, the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, then rinsed by washing solution to remove protein and residual guanidinium salt, etc., and finally eluted to get purified nucleic acid, and the obtained nucleic acid product can be used in experiments such as PCR amplification, nucleic acid detection and analysis, gene mutation detection, and sequencing.

Advantages

1. Lysis and binding with one step, after adding the sample can be on the machine for extraction, the extraction process does not need to pause to add other reagents.

2. The extracted nucleic acid has high purity and good integrity.

3. Efficient and quick, with automatic nucleic acid extractor, only 9 minutes to complete the extraction process.

4. No need to add proteinase K in the extraction process, and it can be transported and stored at room temperature.

5. The reagent system has good accuracy, good reproducibility and high sensitivity.6、Strong versatility, can be used with different manufacturers of automated nucleic acid extraction equipment.

Experimental Case

A coronavirus pseudovirus was extracted on a fully automated nucleic acid extractor LJbio32H using swab-based nucleic acid extraction reagents from Shanghai Leader Biotechnology Co. The extracted products were subjected to qPCR, and the data related to the precision and sensitivity were as follows:

The results showed that the amplification curves of both high and low concentration samples showed good reproducibility. 20 samples with the lowest detection limits could be detected stably.

Application Area

1. Suitable for isolation and purification of nucleic acids from simple samples.

2. Suitable for the isolation and purification of DNA or RNA from minimal content of uncomplicated sample.

3. Suitable for simple and efficient nucleic acid extraction, nucleic acid detection requirements.