Fungal DNA/RNA Extraction Kit 160889

Samples such as mycelium, substrate and spores of fungi release nucleic acid under the action of lysate, and the nucleic acid is separated from other impurities in the sample by the specific adsorption of magnetic beads, and then rinsed by washing solution to remove proteins, polysaccharides, inorganic salt ions, etc., and finally, after elution, purified nucleic acid is obtained, and the nucleic acid product obtained can be used for PCR, nucleic acid detection and analysis, molecular hybridization, gene mutation detection and sequencing and other experiments.

Advantages

1. The core raw materials are produced by ourselves, product quality and yield are guaranteed.

2. The purity of the extracted product, high yield and complete electrophoretic bands make it suitable for various downstream applications.

3. Magnetic bead method of extraction, can be automated, about 20 minutes to complete the operation.

4. Safe to use, the product does not contain harmful reagents such as phenol and chloroform. Unique buffer system ensures reproducibility.

5. Versatile and suitable for extracting nucleic acids from a wide range of fungal samples such as mycelium, ascospores and spores.

Experimental Case

Case: Different fungi were sampled and DNA/RNA was extracted with the following results:

Number	Sample	A260/A280	A260/A230	Conc.(ng/ul)	Yield（μg）
1	20mg Mushroom	1.901	1.905	147.75	14.775
2		1.908	1.934	149.8	14.98
Average		1.9045	1.9195	148.775	14.8775
Standard deviation		0.0035	0.0145	1.025
CV		0.18%	0.76%	0.69%
3	10mg Needle mushroom	1.864	1.431	73.2	7.32
4		1.869	1.386	72.95	7.295
Average		1.8665	1.4085	73.075	7.3075
Standard deviation		0.0025	0.0225	0.125
CV		0.13%	1.60%	0.17%
5	10mg Yeast dry powder	1.809	1.553	319	31.9
6		1.806	1.548	320.5	32.05
Average		1.8075	1.5505	319.75	31.975
Standard deviation		0.0015	0.0025	0.75
CV		0.08%	0.16%	0.23%

Peak pattern diagram:

Mushroom:

Needle mushroom：

Yeast dry powder:

The electropherogram is shown below:

Result analysis:

The results of the Ultra-Micro Nucleic Acid Assay (UMNA) test showed that:

①A260/A280, A260/A230, the coefficient of variation was below 2%, which proved the high stability of reagent extraction.

②The values of A260/A280 were in the range of 1.8-1.9, and the values of A260/A230 were above 1.4, and the peak shapes were complete and basically overlapped proving that the extracted products were of high purity.

③Nucleic acid extraction yields were above 7 µg for different fungal samples.

The results of agarose gel electrophoresis showed that:

①Different fungal samples with bright bands and good integrity.

In conclusion: the extraction of different fungal samples using fully automated nucleic acid extraction and purification instrument showed high purity and yield of the extracted products, complete electrophoretic bands, and uniform and stable cooperation between the reagents and the instrument.

Application Area

The extracted and purified nucleic acids can be used for PCR, qPCR, enzyme digestion, library construction and Southern blot.