ccfDNA Extraction Reagent 241005

ccfDNA extraction reagent can be used for small circulating DNA (<300bp) in 500 μL-8 mL serum/plasma or urine. The lysis solution not only plays a role in lysing serum/plasma and other samples, but also provides an environment for the magnetic beads to selectively adsorb nucleic acids, thus realizing lysis and binding in one step. The small number of impurities adsorbed by the magnetic beads is removed by washing with the washing solution. The magnetic beads release the adsorbed nucleic acids under the action of the elution solution, and high-quality free DNA (ctDNA, cfDNA, ccfDNA) is obtained. The free DNA extracted by this product does not require toxic reagents such as phenol and chloroform, and the free DNA obtained can be directly used in downstream experiments.

Advantages

• Based on magnetic bead extraction technology, it can process 500 μL-8 mL sample volume, realizing efficient extraction and purification of low concentration samples.
• It can be applied to body fluid samples with low concentration of nucleic acid content, such as serum, plasma, urine, cerebrospinal fluid, pleural effusion, etc.
• Large sample volume (500 μL-4 mL) processing volume, with an elution volume of only 15 μL-200 μL, which is compatible with qPCR, digital PCR and NGS platforms.
• High throughput: it can be operated manually or automated, and can be operated with a single tube or with a 24-channel automatic nucleic acid extractor to extract 24 samples at the same time.
• When used with the automatic nucleic acid extractor, free DNA can be isolated from 24 samples in 15-30 min.
• Room temperature lysis combined with one-step, simple operation, the resulting DNA is not easy to degrade.
• Safe and non-toxic, does not contain phenol and chloroform and other toxic reagents.

Application fields

• Liquid biopsy field, circulating tumor cells (CTC), circulating tumor DNA (ctDNA), circulating free DNA (ccfDNA) in peripheral blood or other body fluids, so as to achieve the purpose of diagnosis or monitoring of tumors .
• It can be used in the fields of tumor screening, prognosis evaluation, treatment effect evaluation and personalized treatment .

Supplier Introduction

Shanghai Lingjun Biotechnology Co., Ltd. was established in 2016 and is a professional manufacturer of biomagnetic materials and nucleic acid extraction reagents.

We have rich experience in nucleic acid extraction and purification, protein purification, cell separation, chemiluminescence and other technical fields.

Our products are widely used in many fields, such as medical testing, genetic testing, university research, genetic breeding, and so on. We not only provide products but can also undertake OEM, ODM, and other needs. If you have related needs, please feel free to contact us at Julie.Leung@lingjunbio.com.